Proper use of the Transfer Pipette can help extend the life of the Transfer Pipette, improve pipetting accuracy and maintain user health. Here are some common mistakes in using labs, I hope you’ll be more careful after reading:
Wrong immersion depth
Correct immersion depth for the pipette tips can improve accuracy by up to 5%. The pipette tip should be submerged 1-2 mm for micro-volume pipettes and a maximum of 3-6 mm for normal volume pipettes, depending on the pipette tip size. If the pipette tip is submerged too deeply, the volume of gas in the pipette tip will be compressed, causing too much liquid to be drawn in.
Wrong pipetting angle
The immersion angle of your pipette tip in the sample should be as vertical as possible and should not deviate more than 20 degrees from vertical. A more horizontal angle will draw too much liquid into the pipette tip, resulting in inaccurate aspiration. For example, at an angle of 30 degrees to vertical, up to 0.7%, too much liquid can be drawn in.
Greater accuracy and sample-to-sample reproducibility is possible when each sample is dispensed to the last drop and nothing sticks to the pipette tip. Dispensing with the tip of the pipette against the wall of the container is recommended for most applications as this will reduce or eliminate the amount of sample remaining in the pipette tip. This technique can improve accuracy by 1% or more.
Do not pre-rinse
When dispensing liquid with a pipette, a small film of the liquid remains on the pipette tip, so the expected volume is slightly less than it should be. Pre-rinsing a new pipette tip with the liquid to be used at least twice will condition the inside of the pipette tip.
Irregular Pipetting Rhythm
Maintain a consistent pipetting rhythm as you move from sample to sample. Make sure you are not in a rush and take the different steps slowly. Maintain a good rhythm during each step of the pipetting cycle.
In order to facilitate the process of aspirating the sample into the suction pipe in the situation, the Transfer Pipette tips will maintain a 45° dip or even closer to the horizontal suction while doing so. But it was wrong.
1) When the sample is moved closer to or reaching the maximum volume of the tip, the Transfer Pipette is tilted at a large angle so that the liquid slips into the handle of the Transfer Pipette, causing contamination or even destroying the transfer piston pipette, causing cross-contamination of the sample;
2) The large angle of the Transfer Pipette also increases the contact between the outer wall of the suction pipe and the liquid surface, so that when the suction head leaves the liquid surface, the liquid still on the outer wall is also more, it is easy to dispense ARGUE with the tip the liquid moved away from the tip, reducing the pipetting accuracy, which is likely to be lethal for the dilution of the fine liquid (e.g. 2ul and 10ul) transfer pipettes.
In the laboratory, the user often sees “efficiency” in the liquid when the thumb quickly releases, which makes the liquid quickly rush to the inside of the tip. As a matter of course, this forces the liquid to form a turbulent state after entering the tip, resulting in the invisible vapor mist entering the Transfer Pipette within the SMB range Transfer Pipette, and for a large range of Transfer Pipettes, You will see the liquid rushed directly into the transfer pipette. The end result is contamination and even corrosion of the piston of the Transfer Pipette, easily leading to cross-contamination of the sample, the accuracy also affects.
A large number of users in the transfer pipette after using the transfer pipette directly on the bench will be placed as a clean neat can the transfer pipette in the tray. This is actually not good. Pipetting while doing so, it is inevitable that there will be liquid inside the transfer pipette, in the flat Brazilian, the liquid will remain in the piston and other components, over time after the contamination of the piston and even corrosion of the piston.