Western blotting is a technique that is specifically used for protein analysis. The western blotting technique initiates with separation of molecules using gel electrophoresis. At the end of electrophoresis the separated molecules are transferred to a second matrix which commonly includes polyvinylidene difluoride (PVDF) or nitrocellulose membrane. Then, to prevent any nonspecific binding of antibodies to the surface of the membrane it is blocked with a specific mechanism.

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